PyBOF is a Python library that enables in-memory loading and execution of Beacon Object Files (BOFs) through byte manipulation. The library allows users to load BOF files and execute specific BOF functions within a Python interpreter environment. The tool supports various argument passing methods including raw strings and packed/formatted arguments, with the ability to specify target functions for execution. PyBOF includes warning mechanisms for BOF functions that do not return values, providing feedback during execution. Built on a C extension foundation, the library integrates into Python environments through standard installation and import procedures. It provides a programmatic interface for BOF manipulation and execution, enabling automation and integration with existing Python-based security workflows.